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KMID : 0545120130230060833
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 6 p.833 ~ p.836
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Enhancement of Supercritical CO2 Inactivation of Spores of Penicillium oxalicum by Ethanol Cosolvent
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Park Hyong-Seok
Kim Kyoung-Heon
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Abstract
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The inactivation of spores of Penicillium oxalicum by supercritical carbon dioxide (SC-CO2) was optimized by response surface methodology. The optimal inactivation conditions of 16.8 MPa, 49oC, and 20 min were determined using ridge analysis, at which the predicted and experimental log10 reductions were obtained as 5.74 and 6.12, respectively. The synergistic effect of a cosolvent (ethanol), which was used to modify SC-CO2, on the inactivation of the fungal spores was investigated. At less severe conditions of 10 MPa and 40oC, P. oxalicum spores of 107 CFU/ml were completely inactivated within 45 min by SC-CO2 modified with ethanol.
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KEYWORD
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supercritical carbon dioxide, inactivation, fungal spores, Penicillium oxalicum, cosolvent, ethanol
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